目的 研究SLC30A3在红系分化中的作用.方法 在人髓性白血病K562细胞系中用质粒载体过表达SLC30A3后,检测细胞红系分化指标CD235、ε-、γ-和β-珠蛋白的表达量及细胞增殖速度.流式细胞术检测CD235的表达,荧光实时定量PCR检测珠蛋白mRNA水平,Western blot法检测转录因子GATA-2蛋白水平,MTS法检测细胞增殖速度.结果 过表达SLC30A3使K562细胞的红系分化特异标志CD235的表达升高,CD235阳性细胞率由对照组的34.25％±16.89％增加至95.7％±0.14％ (P＜0.01),ε-、γ-和β-珠蛋白的表达明显升高,红系分化相关转录因子GATA-2的表达降低,细胞增殖速度加快.结论 SLC30A3促进人髓性白血病K562细胞系向早期红系分化.%Objective To study the role of SLC30A3 in erythroid differentiation. Methods After SLC30A3 was over-expressed by plasmid vector in human erythroleukemia K-562 cell line, markers of erythroid differentiation including CD235, ε-,γ-and p-globin, as well as cell proliferation rate were measured. Flow cytometry was used to assess the expression of CD235. Quantitative real-time PCR was used to measure the mRNA level of globins. The protein level of transcription factor GATA-2 was evaluated by Western blot. MTS assay was used to check the cell proliferation rate. Results Over-expression of SLC30A3 up-regulated the expression level of erythroid differentiation specific marker CD235. The percentage of CD235( + ) cells increased to 95.7% ±0. 14% as compared to that of the negative control which was 34.25% ±16.89%(P<0.01). The expression of ε-,γ-and p-globin were also remarkably up-regulated. The expression of erythroid differentiation-related transcription factor GATA-2 was down-regulated. The cell proliferation rate was accelerated as a response to SLC30A3 over-expression. Conclusions SLC30A3 promotes early erythroid differentiation in human erythroleukemia K-562 cell line.
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