人端粒酶反转录酶过表达抑制低氧/复氧大鼠心肌细胞的凋亡

摘要

目的 探讨人端粒酶反转录酶(TERT)在大鼠心肌低氧/复氧(Hypo/Ro)损伤中的保护作用.方法 利用腺病毒将端粒酶催化亚基转染至大鼠 心肌细胞;利用慢病毒介导小干扰核糖核酸(siRNA)沉默心肌细胞基因;研究TERT过表达和沉默后对大鼠心肌细胞Hypo/Ro诱导的细胞凋亡的影响.结 果 Hypo/Ro组细胞凋亡率显著高于对照组;TERT过表达组可显著降低Hypo/Ro心肌细胞的凋亡率;过表达的TERT可降低心肌细胞CytC的表达并增强P21 的表达(P＜0.05).结论 过表达的TERT可抑制Hypo/Ro诱导的心肌细胞凋亡,其机制可能与调控 p21及CytC基因表达有关.%Objective To investigate the effects of human telomerase reverse transcriptase (TERT) on myocardial apoptosis induced by hypoxia/reoxigenation (Hypo/Ro) in rats. Methods Adenovirus mediated transfection of TERT gene into myocardial cells of neonatal rats, lentivirus-mediated siRNA silencing the TERT. To investigate the protective effects of human telomerase reverse transcriptase on myocardial hypoxia/reoxigenation injury in rats. Results The apoptosis rate of Hypo/Ro group was significantly higher than that of the control group. Compared with the Hypo/Ro group, TERT over-expression group significantly reduced the rate of apoptosis, and over-expression of TERT decreased the expression of CytC gene and increased the expression of P21 gene in cardiomyo-cytes (P＜0.05).Conclusions Over expression of telomerase reverse transcriptase inhibits myocardial apoptosis induced by Hypo/Ro. The mechanism may be explained by the regulation to the expression of P21 and CytC genes.