A new triterpenoid saponin, protoprimuloside B (2), has been isolated from the roots of Primula elatior subsp. meyeri and its structure deduced as 3β-O-{[α-L-rhamnopyranosyl-(1-2)-β-D-galactopyranosyl-(1-3)]-[β-D-glucopyranosyl-( 1-2)]-β-D-glucopyranosyl} -protoprimuloside B by means of spectral data, especially NMR (400 MHz), including 1H, 13C, DEPT, COSY,HMBC, HMQC, NOESY techniques and (+)FAB-MS spectrum.
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