3种杀菌剂及其复配剂对斑马鱼的急性毒性

摘要

采用“半静态法”测定了3种农药及其复配剂对斑马鱼的24 h、48 h、72 h和96 h急性毒性,根据我国《化学农药环境安全评价实验准则》中毒性等级划分标准来划分它们对斑马鱼的毒性等级,发现35％精甲霜灵悬浮种衣剂的24 h、48 h、72 h和96 h-LC50均超过10 a.i.mg·L-1,属“低毒”级,而25 g·L-1咯菌腈悬浮种衣剂的24 h、48 h 、72 h和96 h-LC50分别为0.704 mg·L-1、0.514 mg·L-1、0.424 mg·L-1 、0.262 mg·L-1,根据0.100 a.i.mg· L-1 ＜LC50(96 h)≤1.00 a.i.mg·L-1划分,属“高毒”级,80％嘧菌酯水分散粒剂的24 h、48 h、72 h和96 h-LC50分别为9.803 mg·L-1、5.175 mg·L-1、4.328 mg·L 1、2.326 mg·L-1,根据1.00 a.i.mg·L-1＜LC50(96 h)≤10.00 a.i.mg·L-1划分,属于“中毒”级.11％精甲霜灵-咯菌腈-嘧菌酯悬浮种衣剂的24 h、48 h、72 h和96 h-LC50分别为2.267 mg·L-1、2.073 mg·L-1、1.620 mg· L-1、1.280 rmg·L-1,根据1.00 a.i.mg·L-1 ＜LC50(96 h)≤10.00 a.i.mg·L-1划分,属于“中毒”,虽然11％精甲霜灵-咯菌腈-嘧菌酯悬浮种衣剂和80％嘧菌酯水分散粒剂同属“中毒”,但比较具体数值,发现11％精甲霜灵-咯菌腈-嘧菌酯悬浮种衣剂毒性相对更大,原因是其中含有“高毒”的咯菌腈.%Semi-static method was applied to determine the 24 h,48 h,72 h and 96 h-LC50 values of three pesticides and their compounds to Brachydanio rerio.And according to experimental criteria for environmental safety evaluation of chemical pesticides in our country,the 24 h,48 h,72 h and 96 h-LC50 values of 35％ mefenoxam seed coating agent are all above 10 a.i.mg·L-1 so it is of low toxicity.25 g·L-1 fludioxonil seed coating agent is highly toxic because its 24 h,48 h,72 h and 96 h-LC50 values are 0.704 mg·L-1,0.514 mg·L-1,0.424 mg·L-1,0.262 mg · L-1 according to the standard of 0.100 a.i.mg·L-1＜LC50(96 h)≤ 1.00 a.i.mg·L-1.80％ azoxystrobin water dispersible granule is of medium toxicity because its 24 h,48 h,72 h and 96 h-LCs0 values are 9.803 mg·L-1,5.175 mg· L-1,4.328 mg· L-1,2.326 mg· L-1 according to the standard of 1.00 a.i.mg· L-1 ＜LC50(96 h) ≤ 10.00 a.i.mg· L-1.11％ mefenoxam-fludioxonil-azoxystrobin seed coating agent is also of medium toxicity because its 24 h,48 h,72 h and 96 h-LCs0 values are 2.267 mg· L-1,2.073 mg· L-1,1.620 mg· L-1,1.280 mg· L-1 according to the standard of 1.00 a.i.mg·L-1 ＜LC50(96 h)≤ 10.00 a.i.mg· L-1.11％ mefenoxam-fludioxonil-azoxystrobin seed coating agent and 80％ azoxystrobin water dispersible granule are both of medium toxicity.However,considering the LC50 values,11％ mefenoxam-fludioxonil-azoxystrobin seed coating agent is more toxic because it contains fludioxonil.