铅暴露对中华大蟾蜍耳后腺分泌蟾蜍甾烯的影响

摘要

采用半静水式实验方法,研究Pb2+暴露30 d对中华大蟾蜍(Bufo bufo gargarizans Cantor)生理状态和耳后腺分泌蟾蜍甾烯水平的影响.结果表明,随Pb2+浓度(0.68 ～ 5.46 mg·Lˉ1)升高,蜕皮和自发活动减少,存活率下降.进一步采用液相-质谱检测技术发现,0.68 mg·L-1 pb2+暴露下,10种蟾蜍甾烯水平出现1.5倍以上变化.5.46 mg·L-1 Pb2+暴露下,17种蟾蜍甾烯水平发生下调(＞1.5倍),其中bufarenogin水平显著下降(P＜0.05),其他物质水平与对照组无显著性差异.这说明Pb2+暴露对蟾蜍耳后腺中蟾蜍甾烯物质具有潜在影响.%The effect of Pb2+ exposure for 30 days on the physiological status of Bufo bufo gargarizans Cantor and on the levels of bufadienolides in toad venom were studied,using the semi-static water design.With the increase of Pb2+ concentration from 0.68 to 5.46 mg · L-1,the toad's molting and spontaneous activity were remarkably inhibited,and the toad mortality increased.Furthermore,ultra performance liquid chromatography-mass spectrometry was used to monitor the relative levels of bufadienolides in toad venom.We found 10 kinds of bufadienolides with more than 1.5-fold change in 0.68 mg·L-1 Pb2+ exposure group compared with control group.In Pb2+ exposure group (5.46 mg·L-1),17 kinds of bufadienolides were down-regulated with more than 1.5-fold change,in which the levels of bufarenogin was decreased significantly (P＜0.05),while others had no significant change.These illustrated that Pb2+ exposure could affect the levels of bufadienolides in toad venom.