A new resonance light scattering ( RLS) method is used for the determination of α-naphthol in rnurine. In weak alkali medium,a-NAP was oxidized by diacetyl to fonn a-naphthoquinone,which formed a rnfluorescence compound with creatine. Then the fluorescence compound reactsed with CTMAB to form ionic rncomplexes. The intensity of RLS at λmax 580 nm was in proportion of α-NAP concentration in the range of rn1. 44 × 10 -9 ～2. 00 × 10-7 mol/L. The linear regression equation was △I = 186. 9c ( × 10-s mol/L) +rn84. 89 with a correlation coefficient of 0. 9991 , and the limit of detection was 4. 31 × 10-mol/L. The rnmethod had been applied for the determination of α-NAP in urine. The recovenes were in the range of rn96. 0％ ～ 104. 0％ , and the RSD were 0. 80％ ～3. 42％ .%在弱碱性介质中,α-萘酚被双乙酰氧化为α-萘醌,α-萘醌与肌酸生成荧光化合物,该化合物与十六烷基三甲基溴化铵生成离子缔合物,使体系的共振光散射增强,在1.44×10-9～2.00×10-7mol/L范围内,共振光散射强度(R1S)与α-萘酚浓度呈良好线性关系,回归方程为:△I=186.9c(×10-8mol/L)+84.89,r=0.999 1,检出限为4.31×10-10mol/L,样品的加标回收率为96.0%～104.0%,RSD=0.80%～3.42%,该方法可用于尿液中痕量萘酚的测定.
展开▼
