Study on naringenin-CTMAB-DNA system by resonance light scattering technique and its analytical application

摘要

A new high-sensitivity determination method of deoxyribonucleic acid (DNA) with detection limit at nanogram levels was proposed. Based on the measurement of resonance light scattering (RLS), it was found DNA could combine with naringenin and cetyltrimethylammonium bromide (CTMAB) in basic Tris-HCl buffer and produce enhanced RLS signal. The optimum conditions for this system were studied in detail. The enhanced intensity of RLS of naringenin-CTMAB at 353 nm was directly proportional to the concentration of DNA in the range of 0.017-1.7 μg mL~(-1). The detection limit was 5.06 ng mL~(-1). Using the proposed method, the synthetic samples were analyzed with satisfactory results, the recovery was 99.3-105.0% and RSD was 0.7-3.7%.