检测人尿液中尿酸含量的化学发光新方法，试剂稳定、无毒，不使用鲁米诺。在碱性条件下，尿酸选择性地猝灭抗坏血酸与荧光素-十六烷基三甲基溴化铵-铜离子混合物体系的化学发光，尿样直接稀释后化学发光检测尿酸含量。尿酸浓度在0.5~10 mg/L的范围内与化学发光信号具有良好的线性关系，线性回归方程为ΔI =306.6 c+10.904（n=5，R2=0.990），最低检出限为0.06 mg/L（3σ），定量检出限为0.2 mg/L（10σ），对浓度1 mg/L的尿酸平行测定n=11，RSD为1.2%。将本法用于人体尿液中尿酸含量的测定，与临床测定结果进行了对比，并进行了回收率实验，说明本方法具有较好的准确性和选择性；结合流动注射技术操作简便，对临床检验有参考价值和转化应用潜质。%Development of new luminol free chemiluminescence method for determination of uric acid in human urine with the use of non-toxic and stable chemiluminescent reagents. In alkaline solution,chemi-luminescence produced by ascorbic acid reacting with fluorescein-CTMAB-copper ion can be selectively quenched by the presence of uric acid,diluted urine samples were analyzed by proposed chemilumines-cence method. The uric acid concentration in the range of 0. 5 to 10 mg/L and chemiluminescence signal has a good liner relationship,obeyed the equation △I=306. 6 c+10. 904(n=5,R2 =0. 990)with the lowest detection limit of 0 . 06 mg/L( 3σ)and quantification limit of 0 . 2 mg/L( 10σ). The relative standard deviation(RSD)was 1. 2%(n=11)when determining 1. 0 mg/L uric acid standard. By meas-uring the real urine samples in contrast to the clinical results,as well as by the recovery test,the proposed method presented acceptable selectivity,accuracy and sensitivity for the determination of uric acid. In combination with flow injection,the new method achieved simple and robust analytical operation. As such, this method implies its potential as an alternative to be developed for clinical application.
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