Production of Protein Feedstuff by Mixed Fermentation of Waste Lees

摘要

[Objective] To produce protein feedstuff by mix fermentation of waste lees.[Method] Waste lees was fermented by mixture of Aspergillus niger,Trichoderma viride and Candida tropicalis to produce protein feedstuff.The fermentation conditions were optimized by single factor test and orthogonal test.[Result] The optimum fermentation conditions of waste lees are as following:raw materials with fineness of 20 meshes composed of waste lees of 750 g/kg,corn meal of 50 g/kg and wheat bran of 200 g/kg;pH value of inoculum culture,5.0;Aspergillus niger:Trichoderma viride:Candida tropicalis,1:1:1;inoculum size,12%(V/V);fermentation temperature,30 °C;and fermentation time,6 d.After fermentation,the crude protein content,pure protein content,and crude fiber content were increased by 78.97%,56.29% and 31.60%,respectively.In addition,the fermented waste lees was rich in beneficial live microorganisms and bioactive substances such as enzymes and vitamins.[Conclusion] Waste lees can be well fermented to produce protein feedstuff by mix fermentation.