[目的]研究环境水平三苯基锡（triphenyltin，TPT）对哺乳动物肾上腺的毒性作用。[方法]将40只青春期SD大鼠随机分为3个不同剂量的试验组[0.5、5、50μg/（kg·BW）]和1个对照组[0μg/（kg·BW）]。每3 d灌胃1次，暴露54 d后，用10%水合氯醛麻醉并处死大鼠，采用石蜡切片和HE染色的方法观察SD大鼠肾上腺组织学变化特点。[结果]对照组SD大鼠肾上腺组织结构正常，皮质部球状带、束状带、网状带内细胞结构正常，胞核明显；髓质部细胞呈多边形或圆形，排列不规则，索间有血窦。与对照组相比，低剂量组肾上腺皮质和髓质内细胞的结构均无明显变化。中剂量组肾上腺球状带稍变薄，束状带和髓质细胞的胞核固缩、胞质溶解，间质组织轻微出血。高剂量组球状带细胞显著减少，与束状带界限不清，束状带细胞和髓质部细胞胞核固缩，胞质溶解现象加重，呈现细胞空泡化现象，间质组织增厚且出血现象明显。[结论]证实了一定剂量的TPT对SD大鼠肾上腺组织结构具有一定损伤作用，且呈剂量依赖性，该结果为研究TPT对哺乳动物内分泌的影响提供了形态学资料。%Objective] The aim of the present study was to assess the toxic effects of environmental level triphenyltin (TPT) on mammalian adrenal gland. [Methods]Forty adolescent SD rats were randomly assigned to one of four groups:Three TPT treatment groups received intragastric administration with 0.5, 5, 50μg/ (kg·BW) of TPT every 3 days for 54 days respectively and one control group received no TPT. The mice were anesthetized with 10%chloral hydrate and killed. The paraffin sections of the rats adrenal gland tissues were prepared and stained by HE staining method to observe the variation characteristics of their structure. [Results]The tissue structure of adrenal gland of SD rats in the control group was normal; the structure of cells in zona glomerulosa, zona fasciculate and zona reticularis in adrenal cortex was normal, and the nucleus of the cells were limpid;the cells in adrenal medulla were polygonal or circular, the arrangement of the cells was irregular, and there was blood sinus among tissue space. Compared with the control group, there were no significant changes in the structure of the adrenal cortex and medulla in the low dose group. The zona glomerulosa of adrenal in the middle dose group was thinner than that in the lower dose group;there was the nucleus condensation and plasmolysis in the zona fasciculate and adrenal medulla cells, and there was slight bleeding in interstitial tissues. The number of zona glomerulosa cells in high dose group was significantly reduced, and the limit between zona glomerulosa and zona fasciculate was unsharp;the nucleus condensation and plasmolysis in cells in zona fasciculate and adrenal medulla was aggravated, and there was vacuolization in the cells;the interstitial tissues were thicken and bleeding was aggravated. [Conclusion]The results obtained in this study demonstrate that certain doses of TPT can cause damage to the tissue structure of adrenal gland in SD rats in a dose-dependent way. The results also provide morphological data for assessing the effect of TPT on mammalian endocrine function.
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