高效液相色谱法测定混合核苷片中四种核苷的含量

摘要

Objective To establish an HPLC method for the determination of four nucleosides in Mixed Nucleoside Tablets.Methods The separation was performed on C18 column(250 mm ×4.6 mm,5 μm),eluted with 0.01 mol·L -1 potassium dinydrogen phosphate solution and methanol in gradient mode.The flow rate was 1.0 mL·min -1 ,and the detection wave length was 260 nm.With the reten-tion time as for qualitative analysis,the peak area was quantified by the external standard method.Results Four nucleosides were sep-arated well with good linear relation(r =0.999 6).The average recoveries were between 98.4% and 101.9%,and RSD was lower or e-qual to 2.7%.Conclusion The method was simple and accurate,so it can be used for the quantitative and qualitative detection of four nucleosides in Mixed Nucleoside Tablets.%目的：建立高效液相法同时测定混合核苷片中腺嘌呤核苷、鸟嘌呤核苷、尿嘧啶核苷、胞嘧啶核苷含量的方法。方法采用 C18（250 mm ×4．6 mm，5μm）色谱柱；流动相：以0．01 mol·L －1磷酸二氢钾溶液为流动相 A，甲醇为流动相 B，梯度洗脱；流速：1．0 mL·min －1；检测波长：260 nm。用保留时间定性，以峰面积按外标法定量。结果四种核苷分离良好，线性关系良好（r ＝0．9996），平均加样回收率在98．4％～101．9％，RSD％≤2．7％。结论本方法简便、准确，可用于混合核苷片中四种核苷的定性和定量分析。