高效液相色谱法测定复方氯甲霜中氯霉素及甲硝唑的含量

摘要

目的：对复方氯甲霜的质量标准进行再提高研究。方法色谱柱为 AgiLent XDB-C18（150 mm ×4．6 mm，5μm）；流速为1 mL·min －1；柱温为30℃；进样量为10μL；氯霉素的流动相为甲醇－庚烷磺酸钠缓冲溶液（32∶68）；紫外检测波长为277 nm；甲硝唑的流动相为甲醇－水（20∶80）；紫外检测波长为320 nm。结果氯霉素、甲硝唑进样量分别在5．56～111．2，26．02～260．2 mg·L －1浓度范围内与峰面积呈良好的线性关系，r 分别为1．0000和0．9996（n ＝6）；平均回收率分别为103．8％，99．6％，RSD 分别为1．73％和0．43％（n ＝9）。结论该方法简便快速、准确、重复性好，可用于该制剂中氯霉素及甲硝唑的含量测定。%Objective To improve quality standards of Chloramphenicol Metronidazole Cream.Methods Chloramphenicol andmetron-idazole were assayed by HPLC.AgiLent XDB-C18 (150 mm ×4.6 mm,5 μm)was used at the rate of 1.0 mL·min -1 .The injection volume was 10 μL and the column temperature was set at 30 ℃.277 nm and 320 nm were selected asthe detection wavelengths for-chloramphenicol and metronidazole respectively,and themobile phasesweremethanol-sodiumheptanesulfonate(32∶68)forchloramphenicol and methanol-water(20∶80)formetronidazole,respectively.Results A good linearrelationship was established between the peak re-sponse and the concentration of chloramphenicol and metronidazole over the range of 5.56 ~111.2 mg·L -1 (r =1.000 0)and 26.02~260.2 mg·L -1 (r =0.999 6)respectively.The mean recoveries of chloramphenicol and metronidazole were103.8 %(RSD =1.73%)and 99.6 %(RSD =0.43%),respectively.Conclusion The method is simple,accurate and repeatable,which can be used for the determination of Chloramphenicol Metronidazole Cream.