高效液相色谱法测定异叶爬山虎中异槲皮苷的含量

摘要

Objective To establish a method for the determination of Isoquercitrin in EAE extract of Parthenocissus heterophylla (BI.)Merr.Methods The Component was separated on a Inersil C18 column (4.6 mm ×25 mm,5 μm)with a flow-rate of 1 mL·min -1 at25 ℃.The mobile phase consisted a mixture of methanol and 0.05% phos phoric acid (46:54).The total analysis time was 32 min.The detector was DAD and the detection wavelength was 258 nm.Results The linear range of Isoquercitrin was 20 ~200 μg,(r =0.9999).The average recovery was 98.53%,RSD was 0.79% (n =6).Conclusion The method is simple and accurate,and can beused for the determination of content of Isoquercitrin in the EAE extract of Parthenocissus heterophylla (BI.)Merr.%目的 建立高效液相色谱法(HPLC)测定异叶爬山虎中异槲皮苷含量的方法,并测定广西不同地区的异叶爬山虎中异槲皮苷的含量.方法 采用Inersil-C18色谱柱(4.6 mm×25 mm,5μm),流动相为甲醇-0.05%磷酸溶液(46:54),流速1 mL·min-1,检测波长258 nm,柱温25℃,进样量10μL,分析时间32 min.结果 异槲皮苷的进样量在20~200μg范围内,与峰面积呈良好的线性关系,相关系数r=0.9999(n=6);异槲皮苷的平均回收率为98.53%(n=6),RSD为0.79%.结论 该法操作简便,重复性好,可用于测定异叶爬山虎中异槲皮苷含量.