不同剂量的重组人血管内皮抑素腹腔给药对腹水瘤小鼠的疗效观察

摘要

Objective To observe the effects of endostar at different doses by intraperitoneal injection in mice model bearing tumor ascites.Methods The tumor ascites model was established with EAC cells by intraperitoneal injection.A hundred and sixty mice were randomly assigned into 5 groups,32 rats in each group,which were respectively administered with the same volume of NS (control group),12.5 mg · kg-1 endostar,25 mg · kg-1 endostar,50 mg · kg-1 endostar,and 100 mg · kg-1 endostar every other day.The body mass,abdominal circumference and lifecycle of the mice in each group were recorded.The characteristics of ascites were observed and the permeability of peritoneal capillaries was measured.Results (1) Thirteen mice were randomly selected in each group for anatomy at Day 7 and the incidence of ascites was 80％.(2) There was significant difference in body mass between the control group and 100 mg · kg-1 group (P ＜ 0.05),while no difference was found among all the other endostar groups (P ＞ 0.05).(3) Compared with the control group,there were significant differences in abdominal circumference in 50 mg · kg-1 group and 100 mg · kg-1 group (P ＜ 0.05).No differences were found in the other endostar groups (P ＞ 0.05).(4) The degree of bloody effusion decreased with the increased dose of endostar.(5) There were no significant differences in the survival time and OD540 value between each endostar group and the control group (P ＞ 0.05).Conclusions The endostar effect on bloody effusion is dose-related.Compared with other doses,50 mg · kg-1 and 100 mg · kg-1 endostar have significant effect on ascites,which can be regarded as the effective doses of cavity-treatment.%目的 观察不同剂量重组人血管内皮抑素(恩度)对腹水瘤小鼠腹水疗效的差异.方法 建立腹水瘤小鼠模型.将160只小鼠随机分成5组,每组32只,依次给予相同体积的生理盐水(Control组)、12.5 mg·kg-1恩度、25 mg·kg-恩度、50 mg·kg-恩度、100 mg·kg-1恩度,隔天给药.记录各组小鼠的体质量、腹围及生存期,观察腹水的性状,检测腹膜通透性.结果 (1)第7天各组随机解剖13只小鼠检测腹膜通透性,观察到小鼠腹水发生率为80％;(2)体质量方面,仅100 mg·kg-1剂量组与Control组的差异有统计学意义(P＜0.05),余剂量组比较均差异无统计学意义(P＞0.05);(3)腹围方面,对比Control组,50 mg·kg-1和100 mg·kg-1剂量组比较差异有统计学意义(P＜0.05),余剂量组比较均差异无统计学意义(P＞0.05);(4)随着恩度剂量增加,血性积液程度整体上呈减少趋势;(5)与Control组相比,各恩度剂量组的生存期、540 nm波长处Evan蓝的吸光度(OD540)值的比较均差异无统计学意义(P＞0.05).结论 恩度对血性积液的效果与剂量相关.50、100 mg·kg-1剂量组相对其余剂量组腹水抑制作用明显,为恩度腔内治疗的有效剂量.