Objective With CK-19 in the peripheral blood as indicator to assess the role of postoperative chemotherapy and monitoring of blood micrometastases. Methods 120 breast cancer patients involved in the study, nested RT-PCR was used to detect CK-19 mRNA in peripheral blood, and the expression of CK-19 before and after chemotherapy, and the relationship between CK-19 expression and distant metastasis of breast cancer were compared. Results The rate of micrometastasis was 23.33% before chemotherapy, 3.33% after chemotherapy, and the difference was significant(P <0.01); the tumor metastasis rate was 76.9% in CK-19 positive group, 8.6% in CK-19 negative group, and the difference was significant (P <0.01). Conclusion Postoperative chemotherapy can reduce breast cancer micrometastases in blood. CK-19 in peripheral blood can monitor breast cancer micrometastases.%目的 以外周血CK-19为指标评价术后化疗作用及其监控血液微转移意义.方法 以120例乳腺癌术后患者为研究对象,巢式RT-PCR检测外周血CK-19 mRNA,比较化疗前后表达差异及CK-19表达与乳腺癌远处转移关系.结果 化疗前血液微转移阳性率为23.33％,化疗后为3.33％,差异有统计学意义(P＜0.01)；CK-19阳性组肿瘤转移率为76.9％,阴性组肿瘤转移率为8.6％,两组差异有统计学意义(P＜0.01).结论 术后辅助化疗能降低乳腺癌血液微转移；外周血CK-19能监控乳腺癌血液微转移.
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