类风湿关节炎患者治疗前后血清IL-8 IL-10的变化及其临床意义

摘要

目的：检测类风湿关节炎患者（RA）治疗前后血清IL-8、IL-10的变化，探讨其在RA发病机制中的作用及临床意义。方法采用酶联免疫吸附法（ELISA）检测血清中IL-8、IL-10在类风湿关节炎治疗前后的变化，并分析小剂量糖皮质激素治疗的影响。结果① RA患者治疗前与正常对照比较IL-8浓度升高（P＜0．05），治疗后IL-8浓度与治疗前相比降低（P＜0．05），但仍高于正常对照组（P＜0．05）；② RA患者治疗前与正常对照比较IL-10浓度降低（P＜0．05），治疗后IL-10浓度与治疗前比较升高（P＜0．05），与正常对照比较差异无统计学意义（P＞0．05）；③RA组中血清IL-8、IL-10与ESR、CRP、PLT无相关性；④小剂量糖皮质激素治疗后患者血清IL-8浓度明显低于未进行激素治疗的患者。结论 IL-8可能在RA病理过程中起重要作用，可作为监测RA炎症过程的一个有价值指标；血清IL-10在RA发病中可能具有抗炎和免疫抑制作用；小剂量的糖皮质激素可有效抑制RA患者炎症反应。%Objective To detect the change of IL-8 and IL-10 levels in rheumatoid arthritis (RA)before and after treatment,and study its effect and clinical significance in the pathogenesis of RA. Methods Enzyme linked immunosorbent assay (ELISA)was used to de-tect the concentration of serum IL-8,IL-10 before and after the treatment. The changes were also analyzed on the effects of small dose of glu-cocorticoid therapy. Results ①RA patients had higher IL-8 concentration than healthy controls before treatment(P<0. 05). After treat-ment,the concentration was significantly decreased(P<0. 05),but still higher than that of the normal control group (P<0. 05). ②Com-pared with normal control,RA patients had significant lower concentration (P<0. 05). Moreover,treatment increased the concentration of IL-10 in serum (P<0. 05),but there was no statistical difference between treatment group and normal controls (P>0. 05). ③No correla-tion was found between IL-8 or IL-10 and ESR,CRP,PLT in RA group. ④IL-8 concentration in low-dose glucocorticoid treated group was significantly lower than that in non-hormone treatment group. Conclusion IL-8 may play an important role in the pathogenesis of RA,and can be used as a valuable index to monitor the inflammatory process of RA;serum IL-10 may have anti-inflammatory and immunosuppressive effects on the pathogenesis of RA;small dose of glucocorticoid can effectively suppress inflammatory reaction in patients with RA.