吉富罗非鱼mIgD基因恒定区序列的原核表达及多克隆抗体的制备

摘要

实验采用原核表达方法成功获得吉富罗非鱼mIgD基因恒定区融合蛋白，同时利用纯化的MIGD融合蛋白，制备了兔抗MIGD多克隆抗体。ELISA检测MIGD融合蛋白效价高达1∶512000，说明MIGD蛋白具有较强的免疫原性，可以诱导罗非鱼机体产生相应较强的免疫应答。%This experiment adoptsed prokaryotic expression methods to successfully obtain the constant region mIgD gene fusion protein,and polyclonal antibody against mIgD fusion protein was raised in a New Zealand pedigree white rabbit immunized with the purified MIGD fusion protein and the antibodytiter reached 1∶512000.This shows that the MIGD protein has strong immunogenicity,tilapia body can be induced to produce a strong immune response.