高效液相色谱法测定动物组织中的莱克多巴胺残留量

摘要

目的:建立动物组织中莱克多巴胺残留量的高效液相色谱检测方法.方法:采用Symmetry C18色谱柱(4.6m×250 mm,5μm),甲醇-磷酸二氢钾溶液等度洗脱, 流速1.0mL/min,检测器为二级管阵列检测器.结果:目标物保留时间约为6.1min,测定结果在0.1～10mg/L的范围内呈良好的线性(相关系数为0.9997),RSD<5.0%,检出限为10μg/kg,在猪肉样品中3个加标水平的平均回收率为78.1%～90.8%(n=3).结论:该方法操作简单,灵敏度高,选择性好,符合兽药残留分析的要求.%Objective: To establish a high performance liquid chromatographic method to detect content of ractopamine residue in animal tissues. Methods: A Symmetry C18 column (4.6m×250mm, 5μm) was used for separation with methanol-KH2PO4 as mobile phase (1.0mL/min) and a diode array detector for detection. The retention time of ractopamine was 6.1 min. The linear range was 0.1~10mg/L (r=0.9997) with the detection limit of 10μg/kg. The average recovery was 78.1%~90.8% (n=3), and the RSD was ＜5%. Conclusion: The method is simple, sensitive, selective, and qualified for analysis of ractopamine residue in animal tissues.