NaCl胁迫对转甜菜碱醛脱氢酶基因美丽胡枝子生理的影响

摘要

[Objective] The aim of this study was to explore the effects of exogenous gene introduction on osmotic adjustment of plants. [Method] After pot culture with same time, the transgenic plants expressing betaine aldehyde dehydrogenase gene (BADH) and non-transgenic plants of Lespedeza formosa were treated with different concentrations (0, 0.5%, 1.0%, 2.0%) of NaCl solution for 30 h and some physiological indices including proline, betaine, soluble sugar, malondialdehyde (MDA) contents and superoxide dismutase(SOD) activity were assayed. [Result] There was no significant difference on the indices mentioned above between transgenic plants and non-transgenic plants under the salt-free treatment. With the increase of NaCl concentration, the transgenic plants accumulated much higher level of proline, betaine, soluble sugar and lower level of MDA than non-transgenic plants. But the differences of SOD activity increase degrees between transgenic and non-transgenic plants were not significant. Performances of the three transgenic lines were different to some extent. [Conclusion] The introduction of exogenous BADH gene enhanced the osmotic adjustment ability of L. formosa, furthermore; different transgenic lines performed differently.