首页> 外文期刊>农业科学与技术(英文版) >NaCl胁迫对转甜菜碱醛脱氢酶基因美丽胡枝子生理的影响
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NaCl胁迫对转甜菜碱醛脱氢酶基因美丽胡枝子生理的影响

机译:NaCl胁迫对转甜菜碱醛脱氢酶基因美丽胡枝子生理的影响Effects of NaCl Stress on Some Physiological Indices of Transgenic Lespedeza formosa Expressing BADH Gene

摘要

[Objective] The aim of this study was to explore the effects of exogenous gene introduction on osmotic adjustment of plants. [Method] After pot culture with same time, the transgenic plants expressing betaine aldehyde dehydrogenase gene (BADH) and non-transgenic plants of Lespedeza formosa were treated with different concentrations (0, 0.5%, 1.0%, 2.0%) of NaCl solution for 30 h and some physiological indices including proline, betaine, soluble sugar, malondialdehyde (MDA) contents and superoxide dismutase(SOD) activity were assayed. [Result] There was no significant difference on the indices mentioned above between transgenic plants and non-transgenic plants under the salt-free treatment. With the increase of NaCl concentration, the transgenic plants accumulated much higher level of proline, betaine, soluble sugar and lower level of MDA than non-transgenic plants. But the differences of SOD activity increase degrees between transgenic and non-transgenic plants were not significant. Performances of the three transgenic lines were different to some extent. [Conclusion] The introduction of exogenous BADH gene enhanced the osmotic adjustment ability of L. formosa, furthermore; different transgenic lines performed differently.
机译:[目的]本研究的目的是探讨外源基因引入对植物渗透调整的影响。 [方法]在具有相同时间的盆栽培养物之后,用不同浓度(0,0.5%,1.0%,2.0%)NaCl溶液处理表达甜菜碱醛脱氢酶基因(BADH)和非转基因植物的转基因植物。测定30小时,包括脯氨酸,甜菜碱,可溶性糖,丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性的一些生理指标。 [结果]在无盐处理下转基因植物与非转基因植物之间的上述索引没有显着差异。随着NaCl浓度的增加,转基因植物的脯氨酸植物累积了更高水平的脯氨酸,甜菜碱,可溶性糖和低水平的MDA,而不是非转基因植物。但转基因和非转基因植物之间的SOD活性增加的差异并不重要。三种转基因系的性能在一定程度上不同。 [结论]外源性BADH基因的引入增强了L. Formosa的渗透调节能力;不同的转基因系不同地进行。

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  • 来源
    《农业科学与技术(英文版)》 |2008年第6期|146-150|共5页
  • 作者单位

    北京林业大学林木花卉遗传育种教育部重点实验室,北京,100083;

    北京农学院园林系,北京,102206;

    北京林业大学林木花卉遗传育种教育部重点实验室,北京,100083;

    华南农业大学林学院,广东广州,510642;

    北京农学院园林系,北京,102206;

    北京农学院园林系,北京,102206;

    北京农学院园林系,北京,102206;

    北京农学院园林系,北京,102206;

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