蜡样芽孢杆菌JK14 对小麦全蚀病的防治及其抑病机理

摘要

[Objective]Study on control efficacy and inhibitory effect d Bacillus cereus strain JK14,against wheat take-all disease,investigating its antifungal mechanism.[Method]B.cereus JK14 was isolated from soil in the rhizosphere of wheat,inhibitory effects of whose nutrient solution form against Gaeumannomyces graminis var tritici stroins 9862 and 9812 were measured in laboratory and then for its antifungal mechanism.The strain JK14* with rifampicin and wheat take-all disease resistance was screened by increasing concentration of the two substrates,and colonization of JK14* was studied.[Result]In pot experiment,the control effects of JK14* against 9862 and 9812 are 63% and 59%,respectively,which are higher than that of chemical fungicides,with 55% and 51%,respectively.JK14* could deform mycelium and causes degradation of cell wall.And there are also dynamic change of JK14* in root system.JK14* on seed could extend to root along with seed germination and rooting,but per unit tissue mycelium number decreased gradually.[Conclusion]The results indicate some control efficacy of B.cereus strain JK14 against wheat take-all disease.