赖氨酸对绵羊GHR基因表达的影响

摘要

[Objective] This study was to investgate the effects of lysine on the growth of sheep and its mechanism. [Method] Fifteen sheep about one-year old as experimental material were divided into three groups (group A, group B and group C), into whose basal feed 0, 4 and 10 g lysine were respectively added. After 28 d of feeding, the experimental sheep were all slaughtered for sampling; then total RNAs were extracted from the samples and used to clone GHR and GAPDH genes via retrotranscription for analyzing the expression abundance of GHR mRNA in longissimus dorsi muscle from different treatments. [Result] The expression of GHR mRNA in treatment B was significantly higher than that in treatment A(P<0.01), and significantly higher than that in treatment C (P<0.05); while that in treatment C and treatment A was insignificantly different(P>0.05). [Conclusion] Addition of lysine into basal feed of sheep could dose-independently improve the expression of GHR gene in longissimus dorsi muscle.