蔗糖和锌对水稻根系生长的调节

摘要

[Objective] The aim was to study the relationship between urcrose, zinc and the root system growth in rice. [Method] Changes of root system growth, ROS generation and root system proton export ability were analyzed in rice （Oryza sativa L. cv Zhonghua No.11） treated with different concentrations of Zn （NO3）3 sucrose, com- bined sucrose and Zn （NO3）3 mannitol as well as mannitol plus Zn （NO3）2. [Result] The results showed that treatment with 1-3 mM Zn（NO3）2 resulted in significant increases in total root length /number and in accumulation of H202 and 02- but decreases in root system proton export ability. With the exception of shoot length, the length of primary, adventitious, and lateral roots, and the number of adventitious, and lateral roots on primary /adventitious roots were all influenced by different concentrations of sucrose. High concentrations of sucrose caused increases in H202 and O2-, starva- tion or high concentrations of sucrose reduced root system proton export ability after treating with or without Zn. However, at the same concentration of sucrose, different changes of these indicators were observed between Zn and non-Zn treatments. The regulation of root system growth induced by sucrose was marked different from that of mannitol at the same concentration of 5%, suggesting that these effects were caused by sugar signal but not by osmotic potential. [Conclusion] This study indicat- ed that both sucrose and Zn play important roles in the regulation of rice root system growth.%【目的】研究锌和蔗糖与水稻根系生长发育的关系。【方法]以水稻中花11号为试验材料，分析了不同浓度的锌、蔗糖、甘露醇、锌＋蔗糖和锌＋甘露醇处理条件下水稻根系生长、活性氧产生和根系质子分泌的变化。[结果]在1～3mmol／L范围内随着锌浓度的增加，水稻根系总长度、数量及活性氧的产生均增加，但质子分泌水平降低。除了株高外，初生根长、初生根侧根长／数、不定根长，数和不定根侧根长傲都不同程度的受到蔗糖浓度的影响，高浓度蔗糖诱导活性氧增加，无糖或高浓度蔗糖都导致根系质子分泌能力降低。但是，在相同浓度的蔗糖处理条件下锌诱导这些指标的变化与无锌处理的明显不同。5％蔗糖和5％甘露醇对根系生长的调节有显著差异，说明该调节作用是由蔗糖信号而非渗透势引起的。[结论1该研究表明蔗糖和锌对水稻根系的生长都具有重要的调节作用。