人工感染鸡传染性喉气管炎的病理组织学研究

摘要

[Objective] This study aimed to investigate the histopathological changes in chickens artificial y infected with infectious laryngotracheitis(ILT). [Method] Through ar-tificial injection of al antoic liquid containing ILTV into chickens to induce infectious laryngotracheitis, the clinical symptoms in infected chickens were observed; tissues of trachea, lungs, heart, liver, kidney and spleen were col ected from dead chickens, and prepared into paraffin sections, fol owed by histopathological observation under a microscope. [Results] This disease occured in the inoculated chickens 3d later, with a morbidity rate of 95%. The main symptoms were dyspnea, asthma and coughing up of bloody exudate; yel owish-white pseudomembrane was observed on dissected larynx trachea; swel ing, haemorrhage, and further erosion were observed on the in-fected tracheal mucosa, resulting in death of chickens, with the mortality rate of 25%; histopathological observation showed that parenchymal organs exhibited exuda-tive inflammation; swel ing, degeneration, necrosis and shedding of epithelial cells were observed; disturbance of blood circulation occured. [Conclusion] This study pro-vides reference bases for the treatment of ILT.%[目的]研究人工感染鸡传染性喉气管炎（ILT）的病理组织学变化。[方法]采用人工注射含传染性喉气管炎病毒鸡胚尿囊液的方法诱发鸡传染性喉气管炎，观察感染鸡的临床症状，同时分别取病死鸡的气管、肺脏、心脏、肝脏、肾脏和脾脏，采用石蜡包埋、HE染色法，在显微镜下进行病理组织学观察。[结果]接种鸡3 d后发病，发病率为95%，主要症状为呼吸困难、气喘、咳出血样渗出物，剖检以喉头气管有黄白色假膜为特征，受侵害的气管粘膜发生肿胀、出血，从而导致糜烂，以致鸡死亡，死亡率为25%；病理组织学观察表明：实质器官为渗出性炎症；上皮细胞发生肿胀、变性、坏死、脱落；发生血液循环障碍。[结论]该研究为治疗ILT提供了参考依据。