辣椒杂交种纯度鉴定的 SSR核心引物筛选

摘要

Objective] This study aimed to screen a set of SSR core primers suitable for purity identification of pepper (Capsicum) hybrids. [Method] DNA fingerprint of 100 pepper hybrids was analyzed using 17 SSR primers. [Result] According to the polymorphism and heterozygosity, Hpms1-214, Es395 and Hpms1-5 were determined as three preferred core primers for purity identification of pepper hybrids. By using these three preferred core primers, 97 pepper hybrids (accounting for 97%) had heterozygous band pattern with at least one primer. Es330, Es363, Epms923, Es120 and Es64 were determined as candidate core primers for purity identification of pepper hybrids. Specific primers of 14 varieties were obtained, which could be used to further screen parent-complementary primers of each pepper hybrid. [Con-clusion] This study laid the foundation for constructing standard DNA fingerprints for purity identification of pepper hybrids.%[目的]该研究旨在筛选一套适于辣椒杂交种纯度鉴定的核心SSR引物。[方法]利用17对 SSR引物对100份已知辣椒杂交种进行DNA指纹分析。[结果]根据多态性和杂合率两个指标，确定 Hpms1-214、Es395和Hpms1-5为辣椒杂交种纯度鉴定的首选核心引物。利用这3个引物进行筛选，97个品种(占97%)能够找到具有杂合带型的鉴定引物，确定5个引物 Es330、Es363、Epms923、Es120和 Es64为辣椒杂交种纯度鉴定的备选核心引物。筛选出14个品种的特异性引物，可进一步筛选每个辣椒杂交种的双亲互补型引物。[结论]该研究为构建辣椒杂交种纯度鉴定标准DNA指纹图谱奠定基础。