臭氧胁迫对矮化自根砧木M9T337生理生化特性的影响

摘要

[Objective] To investigate the effects of different ozone concentrations (30,60, 80, 100 and 120 μg/L) on the physiological and biochemical characteristics of M9T337, so as to provide theoretical basis for breeding anti-ozone dwarfing rootstocks.[Method] The effects of different concentrations of ozone on chlorophyll content,antioxidant enzyme activity, malondialdehyde (MDA) and soluble protein content of M9T337 were studied by potting dwarfing rootstock M9T337 as test material.[Result] With the increase of ozone concentration, the content of chlorophyll in M9T337 leaves decreased gradually. The activity of SOD (superoxide dismutase)and CAT (catalase) increased first and then decreased, while POD (Peroxidase) activity and MDA (malondialdehyde) content were gradually increased. [Conclusion]The comprehensive analysis of the indicators indicated that the potted M9T337 could tolerate the low concentration (<80 μg/L) ozone stress in the short time (<3h), and it would cause serious injury to M9T337 when the ozone concentration exceeded 80 μg/L.%[目的]研究不同臭氧浓度(30、60、80、100和120μg/L)对M9T337生理生化特性的影响,为培育抗臭氧矮化砧木提供理论依据.[方法]以盆栽矮化自根砧木M9T337作为试材,通过OTC气室中处理3 h后,研究不同浓度臭氧对M9T337叶绿素含量、 抗氧化酶活性以及丙二醛和可溶性蛋白含量的影响.[结果]随着臭氧浓度的升高,M9T337叶片中叶绿素的含量逐渐降低;SOD(超氧化物歧化酶)、CAT(过氧化氢酶)的活性成先升高后下降的趋势,而POD(过氧化物酶)的活性与MDA(丙二醛)的含量均逐渐升高.[结论]综合分析各项指标表明,盆栽M9T337幼苗可以忍受短时间(﹤3 h)内低浓度的(﹤80μg/L)臭氧胁迫,当臭氧浓度超过80μg/L时会对M9T337产生严重的伤害.