广谱持久抗病基因Pi40在云南高原粳稻的应用

摘要

对持有主效广谱抗瘟基因Pi40的单基因系4163与其它11份单基因鉴别品系进行自然条件下稻瘟病抗性鉴定.结果表明,4163在云南省粳稻区的稻瘟病重病区均表现抗病,即在保山、宜良、玉溪叶瘟田间抗性分别为0级、0级、4级,穗瘟田间抗性为3级.同时,利用49份有代表性的云南稻瘟病单胞菌株,通过室内接种抗性鉴定方法建立了Pi40对云南稻瘟病的抗谱,并与另外25份抗稻瘟病单基因系进行室内接种同步鉴定比较.结果表明,Pi40对49份云南地方稻瘟病菌株的抗病频率为87.8％,表现出较广的抗谱,可以作为云南高原粳稻抗病育种的新抗源利用.%The resistance of a newly bred line 4163 containing the rice blast resistance gene Pi40 and another 11 monogenic lines to rice blast in natural disease nurseries was identified.The results showed that 4163 was resistant to rice blast isolates from Yunnan,and the leaf blast resistance levels were at grades 0,0 and 4 in Baoshan City,Yiliang City and Yuxi City respectively,while the ear blast resistance was at level 3.Meanwhile,the resistance spectrum of Pi40 to 49 representative rice blast isolates from Yunnan Province was studied by indoor inoculation and was compared with another 25 resistance genes.The results revealed that the resistance frequency of Pi40 to the 49 typical isolates reached 87.8％,showing a broader resistance spectrum,so it could be used as a new source of resistance genes in breeding of japonica rice in Yunnan plateau in the future.