水稻稻瘟病广谱抗病新等位基因pi21 t的鉴定及其抗性应用

摘要

为不断发掘和鉴定新的抗性基因,从而克隆和利用广谱持久抗性基因,选育水稻广谱抗稻瘟病新品种,解决稻瘟病危害。根据已克隆的水稻稻瘟病广谱抗病隐性等位基因pi21的序列设计分子标记Pi21-1,对育种上高频率使用的广亲和品种02428进行PCR扩增,测序比较分析发现了新的等位基因类型,同时对广亲和品种02428进行田间稻瘟病接种鉴定,苗期鉴定结果为免疫,从而鉴定了一个广谱抗稻瘟病的新等位基因pi21t,为水稻广谱抗稻瘟病育种提供了新的有利资源。通过分子标记辅助选择,能够快速明确目标品种中所携带目标基因的等位基因型,从而加快水稻广谱抗稻瘟病育种的进程,提高抗病效率。%In order to discover, identify and apply the new broad-spectrum resistant genes, this research had identified one new rice blast broad-spectrum resistant allele. In this study,according to the sequence of rice blast broad-spectrum resistance recessive allele pi21 , molecular marker Pi21-1 was designed and amplified using wide compatibility rice variety 02428. Sequencing analysis showed new mutation type,and rice blast vaccination identifi-cation resulted in immunity. Thus,a new rice blast broad-spectrum resistance allele pi21t had been identified. These results provided new resistant resources for rice blast broad-spectrum resistant breeding. Meanwhile,genotype of tar-get variety could be determined rapidly using newly designed marker. All these accelerate the process of rice blast broad-spectrum resistant breeding.