Resistance Selection Against Abamectin in Tetranychus cinnabarinus (Boisduval) and Changes in Its Detoxification Enzyme Activity

摘要

The susceptible strain of Tetranychus cinnabarinus (Boisduval) was collected from the cassava germplasm resource garden of Guangxi South Subtropical Agricultural Science Research Institute and raised for 15 generations,during which resistance selection against avermectin was performed through the indoor spraying of avermectin,thereby obtaining an avermectin-resistance strain (Ab-R) with a resistance ratio of 3.25.After the indoor resistance selection,the determination of detoxification enzyme activity in the Ab-R and SS strains showed that the specific activity of carboxylesterases (CarE),glutathione-s-transferases (GSTs) and multi-functional oxidase (MFO) in Ab-R15 was 1.27,1.69 and 1.92 times of that in SS,respectively.In addition,the specific activity of MFO in Ab-R5,Ab-R10 and Ab-R15 was significantly different from that in SS;there was no significant difference in the specific activity of CarE compared with SS when screened to the F10 generation;and when screened to the F5 generation,there was no significant difference in the specific activity of GSTs compared with SS.The results showed that the significant increase in MFO activity was an important cause of resistance to avermectin in T.cinnabarinus,and CarE and GSTs were also involved in the formation of avermectin-resistant strains.