Study on Method for Determination of Artemether Content in New Artemether Injection

摘要

This study was conducted to establish a high performance liquid chromatography( HPLC) method for the determination of artemether in the artemether injection using Hypersil ODS C18 chromatographic column( 5 μm,4. 6 mm × 150 mm). Mobile phase,column temperature,flow rate and detection wavelength were optimized. Acetonitrile-water-tetrahydrofuran( 62∶ 37∶ 1,V/V) was selected as the mobile phase,and the HPLC was performed with column temperature at30 ℃ and the flow rate at 1. 0 ml/min; and the detection wavelength was set at 216 nm. The HPLC detection system of artemether had good suitability. The linearity was good in 100-800 μg/ml concentration range,and the regression equation was y = 302. 36 x-682. 02,R2= 0. 999 8. The overall average recovery was97. 58%,and the RSD was 1. 58%. Three batches of artemether injection samples were determined by the method,showing RSD of 1. 42%. The method could be used for the detection of artemether content in artemether injection.