Analysis of Cloning and Expression Characteristics of Capsicum chinense Jacq. CcMYB Gene

摘要

cqvip:In order to discuss the role of MYB gene in capsaicine synthesis process,one CcMYB gene was cloned from Capsicum chinense Jacq. by RT-PCR. Its cDNA has a total length of 1 038 bp,and was speculated to code 345 amino acids,comprising an complete open reading frame. The isoelectric point is 8. 57,and the molecular weight is 38. 2 KD. The protein is a neutral hydrophobin without transmembrane structure. There are two MYBDNA domains at the N terminal. The fluorescence quantitative PCR results showed that CcMYB gene was expressed in all the root,stem,leaf,flower,placenta and fruit tissue of pepper,and the expression level was the highest in fruit; and CcM YB was expressed in fruit at the highest level at turning stage,and at the second highest level at expansion stage,which accords with the expression profile of pun1 gene in fruit development period. It is speculated that CcMYB gene plays an important role in the regulation of capsaicine synthesis in C. chinense fruit.