Acute Toxicity Test of Miantan Fuzheng Powder

摘要

[Objectives]An acute toxicity test of Miantan Fuzheng Powder was conducted on rabbits,which were given Miantan Fuzheng Powder at its maximum administration dosage and observed for 14 d,so as to provide reference for medicine development and clinical test.[Methods]New zealand rabbits were divided into four groups randomly,each of which included 4 rabbits,half male and half female.The Miantan Fuzheng Powder-intact skin and Miantan Fuzheng Powder-damaged skin groups were applied with Miantan Fuzheng Powder once at a dose of 5 g/kg body weight,and the blank control group-intact skin and blank control-damaged skin groups were given the auxiliary material(vaseline)once.The rabbits were observed continuously for 14 d after the administration,and variation in body weight was recorded during the observation and then statistically analyzed with SPSS.After the 14 d of observation,the animals were killed through pentobarbital anesthesia and anatomized to observe main visceral organs including heart,liver,spleen lung and kidney.If toxic effect or organ abnormality exists,pathological sections would be prepared and observed.[Results]Rabbits in the blank control and Miantan Fuzheng Powder groups all showed normal appearance,behavior,breath state,position and posture,response to stimulation and growth of body weight,and no irritative erythema and edema on the skin,and the damaged skin recovered naturally within 14 d.No obvious abnormality was observed on visceral organs in autopsy.The Miantan Fuzheng Powder-intact skin and Miantan Fuzheng Powder-damaged skin groups had no significant differences in body weight from the blank control group-intact skin and blank control-damaged skin groups before administration,and on the 7 th and 14 th day after administration.[Conclusions]In this acute toxicity test,the Miantan Fuzheng Powder-intact skin and Miantan Fuzheng Powder-damaged skin groups were given the drug at the maximum administration dosage of 5 g/kg body weight,which was about 119 times of the clinical dose for human,and no obvious toxic effect was observed.