[Objective] This study aimed to investigate the enzymatic kinetics of polyphenol oxidase in peel of Nephelium lappaceum to explore the environmental factors affecting its catalytic activity. [Method] With N. lappaceum peel as the experimental material and catechol as substrate, effects of temperature, pH, four inhibitors (EDTA, Vc, sodium bisulfite and citric acid) and substrate concentration on the activity of polyphenol oxidase were investigated. [Result] The optimal temperature for polyphenol oxidase in N. lappaceum peel was 40 ℃, and the optimal pH was 6.8. EDTA, Vc, sodium bisulfite and citric acid all showed ideal inhibitory effects on the activity of polyphenol oxidase; specifically, EDTA had the strongest inhibitory effect. [Conclusion] At low temperature, EDTA, Vc, sodium bisulfite and citric acid with certain concentrations can inhibit the catalytic activity of polyphenol oxidase in peel of N. lappaceum, extend the storage and transportation time of N. lappaceum fruit, and inhibit the browning. This study provides theoretical basis for the development and utilization of polyphenol oxidase in peel of N. lappaceum.
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