Cloning of Immune System-related Gene ApTOLL1 and Its Expression in Nosema-infected Antheraea pernyi

摘要

[Objective]This study aimed to identify the immune system-related TOLL-like receptor family gene of Antherea pernyi,to lay the foundation for further investigating the immune mechanism of Antherea pernyi.[Method] Immune system-related TOLL-like receptor family gene of Antherea pernyi was cloned for sequencing and phylogenetic analysis;in addition,expression variations of TOLL-like receptor family gene in Antherea pernyi infected with Nosema pernyi and Nosema bombycis were detected to analyze the differences in immunological reactivity of Antherea pernyi to Nosema infection.[Result]Based on cDNA cloning and sequencing,an immune system-related gene fragment was isolated from Antherea pernyi,which is the most homologous to the Toll1 gene in Toll signaling pathway of Bombyx mori according to the sequencing result and phylogenetic analysis,which is named ApToll1 gene.Subsequently,Antherea pernyi pupae were injected respectively with Nosema bombycis and Nosema pernyi,fluorescent real-time quantitative PCR analysis showed that ApToll1 was abundantly expressed in Antherea pernyi pupa 2 h after Nosema bombycis injection,which began to express in Antherea pernyi pupa 11 h after Nosema pernyi injection,indicating that the immune response time induced by various Nosema strains varies in Toll signaling pathway of Antherea pernyi.[Conclusion]ApToll1 gene of Antherea pernyi was first cloned in this study,which provides reference for further investigating the immune mechanism of Antherea pernyi.