Screening of Cellulase Producing Fungus ZDTJ097-1-（ 7 ） from Bohai Bay and Its Yielding Conditions

摘要

[Objective] This study aimed to screen a marine-derived cellulase producing filamentous fungus and to investigate the yielding conditions and celluase activity.[Method] A total of 25 marine-derived filamentous fungus were screened out of from Bohai Bay,and one producing cellulase was obtained through Congo red transparent circle method and cellulase activity assay.After that,optimum conditions were examined for cellulase producing and catalyzing.[Result] CMC-Na could induce ZDTJ097-1-(7) strain to produce filter paper cellulase,CMC cellulase and β-glucosidase.The optimum conditions for ZDTJ097-1-(7) to produce enzymes were 0.5% CMC-Na,0.2% NH4NO3,pH 6.0 at 25 ℃.[Conclusion] This study provided reference for further development and utilization of cellulase producing fungus ZDTJ097-1-(7).