[Objective]Peanut in vitro regeneration system was optimized to provide technical support for its genetic transformation and mutagenesis.[Method]The effect of gibberellin,light and genotype on somatic embryogenesis and plantlet regeneration of peanut was studied using Guihua peanut cultivar as materials,leaflets as explants,MS+10 mg/L 2,4-D as somatic embryo induction medium,and MS+3 mg/L 6-BA+0.8 mg/L NAA+(0-15 mg/L)GA3as plantlet induction medium.[Result]The somatic embryo induction rate of five peanut varieties under light condition of light:dark=14 h:10 h was significantly higher compared with that under dark condition by 7.5-37.5 percent points.Among different peanut varieties,Guihua 26 represented the highest somatic embryo induction rate of62.8%,while Guihua 833 represented the lowest somatic embryo induction rate of 21.7%.Adding 5-15 mg/LGA3in plantlet induction medium was conducive to improving the induction rate of plantlets derived from somatic embryos.With addition of 5 mg/L GA3,Guihua 26 and Guihua 771 represented the highest plantlet induction rate of 42.8%and 35.3%,respectively;with addition of 15 mg/L GA3,Guihua 836,Guihua 1026 and Guihua 833 represented the highest plantlet induction rate of 38.7%,33.3%and 26.4%,respectively.Among different combinations of peanut variety and GA3concentration,plantlet induction rate reached the highest in the combination Guihua 26+5 mg/L GA3and reached the lowest in the combination Guihua 836+15 mg/L GA3.[Conclusion]Appropriate light conditions are conducive to peanut somatic embryogenesis in vitro.Adding GA3in plantlet induction medium is conducive to promoting plantlet regeneration.Guihua26 and Guihua 836 are the best peanut varieties among the experimental genotypes for somatic embryogenesis and plant regeneration.
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