A Histological Study on Subacute Toxicity of ＂Baikening＂ in Hy-Line Brown Roosters

摘要

[Objective]A histological study was conducted to investigate the toxicity of"Baikening"and the toxic effect of"Baikening"on roosters after longerterm administration at different dose levels.[Method]Thirty 21-day-old Hy-Line Brown roosters were administered with 0.5 and 1.0 ml of"Baikening"aqueous extract through drinking water once a day for continuous 14 d.Roosters in control group and experimental groups after drug administration for 14 d and drug withdrawal for 7 d were euthanized;livers,kidneys,thymuses,spleens and bursas of Fabricius were collected and weighed for comparative analysis.Various tissues were prepared into paraffin-embedded slices and stained by H.E.,to observe histological changes of various organs under an optical microscope.[Result]After drug administration for 14 d,all the roosters in experimental groups survived;there was no visible pathological change in various organs of Hy-Line Brown roosters in each group;organ weights of Hy-Line Brown roosters in various groups showed no significant difference;observation results of tissue slices indicated that there was no pathological change in fine structures,nuclei and cytoplasm of various organs of Hy-Line Brown roosters in different groups;various blood vessels in different organs also presented no change in blood circulation or other pathological changes.[Conclusion]"Baikening"aqueous extract(oral administration)has no toxic and side effect on roosters in the subacute toxicity experiment.