[Objective] This study aimed to establish a rapid,sensitive and specific method using reverse transcription loop-mediated isothermal amplification(RT-LAMP) technology to detect swine Japanese B encephalitis virus(JEV).[Method] Four specific LAMP primers were designed according to six loci the conservative region of JEV E gene sequence. Positive JEV RNA sample was used as a template for one-step amplification,and the reaction conditions and reaction system were optimized.[Result] Experimental results showed that the established method had high sensitivity,with the detection limit of 0. 5pg; specificity experiments indicated that the method had high specificity and there was no amplification reaction for other viral pathogens. The coincidence rate between detection results of RT-LAMP and RT-Nested-PCR was 90. 9%. After RT-LAMP reaction,a chemiluminescent agent was added for visual observation,which greatly reduced the detection time. This method required no special equipment but only a water bath,which was a simple,sensitive and rapid detection method for swine Japanese B encephalitis virus and could be applied in primary laboratories.[Conclusion] An RT-LAMP detection method for swine Japanese B encephalitis virus was successfully established and preliminarily applied in clinical practice.
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