Regulatory Effects of Xylooligosaccharides on Intestinal Microbial Flora Proliferation and Defecation of BALB/c Mice

摘要

[Objective] This study aimed to investigate the regulatory effects of xylooligosaccharides on intestinal microbial flora proliferation and defecation of BALB/c mice.[Method] In the intestinal microbial flora proliferation regulation experiment, microflora quantities of Bifidobacterium, Lactobacillus, Enterobacter and Enterococci in excrements of BALB/c mice were determined after continuous administration of high-dose[1.0 g/(kg·bw)] and low-dose[0.5 g/(kg·bw)] xylooligosaccharides for 14 d.In the defecation regulation experiment, constipation model of BALB/c mice was established with compound diphenoxylate after continuous administration of high-dose and low-dose xylooligosaccharides for 21 d, to determine the first defecation time of black excrement, weight of black excrements within 6 h and rate of intestinal propulsion of constipation mice.[Result] Compared with the control group, after continuous administration of xylooligosaccharides for 14 d, microflora quantities of Lactobacillus and Bifidobacterium in excrements of BALB/c mice in low-dose and high-dose groups both increased significantly (P<0.05).After continuous administration of xylooligosaccharides, microflora quantities of Enterococci and Lactobacillus in control group were significantly raised (P<0.05 and P<0.01, respectively) compared with those before administration; microflora quantities of Enterococci in high-dose and low-dose groups were significantly reduced (P<0.05), while microflora quantities of Lactobacillus and Bifidobacterium and the B/E value increased significantly (P<0.05, P<0.01 and P<0.01, respectively).After continuous administration of xylooligosaccharides for 21 d, compared with the model group, the first defecation time of black excrement in high-dose and low-dose groups was significantly shortened (P<0.05), the total weight of black excrements within 6 h increased significantly (P<0.05) and the rate of intestinal propulsion was significantly enhanced (P<0.01).[Conclusion] Low-dose (0.5 g/Kg.bw) and high-dose (1 g/Kg.bw) xylooligosaccharides can improve the proliferation of intestinal microbial flora and prevent mice from constipation.