Aiming at solving the existing issues in purity identification of Cucurbita moschata hybrids by SSR,such as complex operation and difficult application in production practice,in this study,a simple SSR-based method was established for purity identification of Cucurbita moschata hybrids. By using the established simple method,without grinding,freezing,centrifugation and drying,the genomic DNA extraction process is shorter than 3 min. Compared with the conventional method,PCR detection system and silver staining in polyacrylamide gel electrophoresis of the established method are more time-saving and cost-saving. The whole detection process is shorter than 4 h,and 480 samples can be detected with this method by one person in one day. In addition,the detection result exhibits a coincidence rate of 99% with field identification. The simple SSR-based method established in this study can provide basis for large-scale rapid purity identification of Cucurbita moschata hybrids.
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