Screening of Primers for DNA Barcoding of Sedum Plants

摘要

[Objective] This study aimed to screen primers for DNA barcoding of Sedum plants. [Method] Sedum lineare Thunb.,S. spectabile Boreau,S. tatarinowii Maxim,S. aizoon L. and S. sarmentosum Bunge were used as experimental materials. Nuclear DNA and chloroplast DNA were extracted from these five Sedum plants with CTAB method for primer screening by PCR amplification. PCR products were detected by agarose gel electrophoresis. [Result] Four sequences suitable for DNA barcoding of Sedum plants were preliminarily screened from six candidate sequences( psb A-trn H,rop B,rbc L,rpo C1,ITS 2,mat K),including psb A-trn H,rop B,rpo C1 and ITS 2. The amplification rate of these four sequences reached 100%. [Conclusion]This study provided basis for DNA barcoding of Sedum plants.