This study aimed to analyze the partial sequence of COI gene in Pengze crucian carp,gold crucian carp,Ukraine carp and common carp,with silver carp and tilapia fish as two outgroups.DNA fragments 814 bp in length were amplified and analyzed.Statistical analysis of sequence compositions and analysis of intraspecific and interspecific genetic distances were performed by using Mega4.1 software.The phylogenetic tree of COI gene was constructed with neighbor-joining method.According to the results,15 fish individuals could be divided into five groups,which was consistent with the results of traditional taxonomy.Furthermore,the partial sequence of COI gene was a good DNA barcode to identify different species and exhibited certain applicability in the analysis of interspecific phylogenetic relationships of Cyprinidae species.
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