Effects of Heat Shock Factor AtHsfAla on Programmed Cell Death in Arabidopsis thaliana under Cold Stress

摘要

[Objective] This study aimed to investigate the effects of heat shock factor AtHsfA1a on programmed cell death in Arabidopsis thaliana under cold stress. [Method] AtHsfA1a-silenced transgenic( NT) and wild-type( WT) A. thaliana seedlings were used as experimental materials to induce the formation of callus; the callus were cultured to single cells by suspension culture,subjected to cold stress,stained with DAPI,prepared into cell smears and observed under a fluorescence microscope. [Result] Under cold stress,cell nucleus of wild-type A. thaliana displayed morphological changes,but no apoptotic bodies were found;apoptotic bodies were observed in AtHsfA1a-silenced transgenic A. thaliana cells,and the cytoplasm was remarkably concentrated. [Conclusion] Under cold stress,heat shock factor AtHsfA1a exerted inhibitory effects on programmed cell death in A. thaliana,which was of great significance for clarifying the mechanism of stress responses in plants.