Investigation of Phylogenetic Relationship among Three Species of Channa Based on ITS Sequence Analysis

摘要

Objective This study aimed to analyze genetic variation of ribosomal ITS region sequences in Channa argus,C. maculata and C. asiatica,and to investigate the phylogenetic relationship among Channa species based on ITS sequences. Method ITS sequences of three Channa species were amplified by PCR,cloned and assembled to obtain the full length of ITS sequences. Result The full length of ITS sequences of C. argus,C. maculata and C. asiatica was 902,927,and 902 /903 bp,respectively. ITS sequences of C. argus,C. maculata and C. asiatica exhibited higher G + C( 72%) than A + T. Interspecific nucleotide differences were significantly greater than intraspecific differences of these three Channa species. Thus,these remarkably differential ITS fragments could be used to identify C. argus,C. maculata and C. asiatica. Phylogenetic tree constructed by Neighbor-joining and Maximum Likehood methods showed that C. argus shared the lowest genetic distance with C. maculata and the highest genetic distance with C. asiatica. [Conclusion] This study provided reference for classification,identification,phylogenetic analysis and interspecific hybridization of Channa species.