Establishment and Optimization of ISSR-PCR Reaction System for Salvia przewalskii Maxim.

摘要

Based on single-factor experiment and L9( 34) orthogonal experiment,a stable ISSR-PCR reaction system of Salvia przewalskii Maxim. was established and optimized. The results indicated that the optimal concentrations of various components in a 20 μl ISSR-PCR reaction system of S. przewalskii Maxim. were: 2μl of 10 × buffer( Mg2 +),0. 4 mmol/L d NTPs,1. 0 U of Taq DNA polymerase,0. 3 μmol/L ISSR primers and 30 ng of DNA template. This study laidthe foundation for the utilization of S. przewalskii Maxim. germplasm resources.