Analysis of DNA Methylation Changes during the Late Fruit Development Stage in Luotian Persimmon

摘要

Persimmon(Diospyros kaki Thunb.),a member of Ebenaceae family,is known as"woody grain"and"iron-stem crop".However,the epigenetic research has been rarely reported.In this study,tannin content in late fruit development periods was determined.Moreover,the MSAP(methylation-sensitive amplified polymorphism)method was used to analyze methylation profile,and 13 primer combinations were selected.In total,5 differential fragments were cloned and sequenced.This research would be helpful for further gene cloning,research on deastringency mechanism,as well as molecular breeding of D.kaki.