Validation of the Red Pericarp Gene from 419 Rice Land- races in Guangxi via Genome-wide Association Studies

摘要

Rice has different colors of pericarp,such as red,white and black. Red rice pericarp is rich in proanthocyanins,which have antioxidant properties and are beneficial to human health. In the present study,we analyzed the red-pericarp gene Rc of 419 rice landraces in Guangxi by genome-wide association study( GWAS),and validated that the Rc gene regulated the red pericarp trait in rice. By analyzing the genomic DNA of 97 red-pericarp rice cultivars,we identified two new alleles in C139 and C323. Then,the exons of Rc^(C139) and Rc^(C323) were sequenced with Sanger method,and the results demonstrated that the natural mutations within Rc gene resulted in the two alleles Rc^(C139) and Rc^(C323).