Isolation and Characterization of Vibrio sinaloensis Penaeus vannamei Boone in Hainan Province

摘要

To effective prevent and control the disease of Penaeus vannamei Boone in Hainan Province,the morphology,cultural characteristics,physiological and biochemical reactions of the dominant strain isolated from the hepatopancreas tissue of diseased P. vannamei were identified by conventional methods,and 16 S rDNA sequence was amplified by polymerase chain reaction( PCR) for sequencing analysis. Furthermore,artificial experimental infection was conducted to detect the pathogenicity of the isolate. Finally,the lytic ability of phage 28^(TM) against the isolate was detected by in-vitro lysis test. The results showed that the isolate( HYRJ2013) was identified as Vibrio sinaloensis. There were similar symptoms with the natural incidence of P. vannamei when the isolate HYRJ2013 was used to infect P. vannamei by muscular injection. The median lethal dose( LD_(50)) was measured as 6. 68 × 10~7cfu/ml. Phage 28 TMcould lyse the isolate and be used for biological control. The results indicate that V. sinaloensis is an opportunistic pathogen to P. vannamei.