In order to find suspension culture conditions suitable for Premna microphylla Turca,with the leaves of experimented P. microphylla as a material,embryogenic callus induction,subculture,dispersion of embryogenic callus and suspension culture were performed. The results showed that the calli induced from leaves were different. The medium most suitable for induction of loose embryogenic callus for suspension culture was MS + 0. 4 mg/L 2,4-D + 0. 4 mg/L NAA + 3% sucrose + 0. 8% agar. A better cell line could be obtained with cellulase and pectinase for dispersion of P. microphylla callus. The medium most suitable for suspension culture was MS + 0. 4 mg/L 2,4-D + 0. 8 mg/L KT + 3% sucrose. In this medium,the fresh weight of the suspension-cultured cells increased by 6. 46 times per 7 d. Dark culture was more suitable for cell proliferation. The average contents of pectin and protein were,respectively,6. 57% and 0. 12% in the suspended cells of P. microphylla,which had been cultured for 3 weeks under the optimum culture conditions.
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