This study aimed to investigate the aseptic treatment and disinfection of E.striatum explants and the effects of three liquid culture medium(PES,PESI,f/2) and plant hormones on adventitious bud induction from explants.The results showed that the vast majority of explants disinfected by combined treatment exhibited good growth and normal color with an average mortality rate of only 9%.E.striatum explants exhibited the best growth in PES liquid medium and the survival rate reached 66.67%at 40 d after culture.Under different concentrations(0.5,2,5 mg/L) of 6-BA,the induction rate of E.striatum explants was significantly higher than that in control group,but no remarkable differences were found in the induction of adventitious buds from E.striatum explants.In PES liquid medium containing 6-BA and 2,4-D,the adventitious bud induction rate was 90%and the average number of induced adventitious buds was 6.63.
展开▼
