基于生存分析模型挖掘HSPB1基因表达与放疗敏感性关系研究

摘要

目的 探讨热休克蛋白(HSPB1) 与胃癌的放疗敏感性关系.方法 采用TCGA 公开的胃癌数据库,随机将胃癌数据等分为2 份,一份为测试组数据,一份为验证组数据.结果 分析结果表明,HSPB1 的表达与胃癌患者的总生存率无显著关联;无论是测试组还是验证组数据,高表达HSPB1的胃癌患者在放疗与非放疗组间总生存差异无统计学意义,多因素调整后也无统计学意义,测试组数据和验证组数据调整后风险比(HR)分别为1. 08(0. 38 ～ 3. 09) 和1. 38(0. 53 ～ 3. 64),P 值分别为0. 89 和0. 51;而低表达HSPB1 胃癌患者经过放疗,效果显著,测试组数据和验证组数据调整后HR为0. 22(0. 06 ～ 0. 81) 和0. 03(0. 003 ～ 0. 220),P 值分别为 0. 02 和0. 001.结论 HSPB1 低表达与胃癌的放疗敏感性存在明显关联,HSPB1 低表达的患者,放疗后生存率大幅上升,提示HSPB1 可作为胃癌精准放疗的潜在有效的分子标志物.%Objective To investigate the relationship between the expression level of heat shock protein B1 (HSPB1) and radiotherapy. Methods We used the gastric cancer data from TCGA. The data was randomly splited to two parts,one as testing data,another one as validation data. Results The results showed that the expression did not associate with overall survival,both on testing and validation data. For patients with high expression of HSPB1,there was no significant different between radiotherapy and nonradiotherapy group. The adjusted HR were 1. 08(0. 38 ～ 3. 09) and 1. 38(0. 53 ～ 3. 64),with P values 0. 89 and 0. 51 for testing and validation data,respectively. Interestingly,for patients with low expression of HSPB1,significant different between radiotherapy and nonradiotherapy group was observed. The adjusted HR were 0. 22(0. 06 ～ 0. 81) and 0. 03(0. 003 ～ 0. 220),with P values 0. 02 and 1. 07 × 10 - 3 for testing and validation data,respectively. Conclusion These results suggest that low expression of HSPB1 strongly associates with radiosensitivity. The survival rate of patients with low expression of HSPB1 after radiotherapy is significantly increased,suggesting that HSPB1 may be a potential molecular marker for precision radiotherapy of gastric cancer.